【英文篇名】 Screening and Identification One Bacteria Producing Cellulase and Partial Characteristics of Cellulase
【作者中文名】 葛春辉; 徐万里; 邵华伟; 张云舒; 孙宁川; 王伟; 余琳;
【作者英文名】 GE Chun-hui1; XU Wan-li1; SHAO Hua-wei2; ZHANG Yun-shu1; SUN Ning-chuan1; WANG Wei2; YU Lin3（1.Institute of Soil and Fertilizer; Xinjiang Academy of Agricultural Science; Urumqi 830091; 2.Xinjiang City Construction and Environmental Protection Co.Ltd; 3.Xinjiang Academy of Environmental Protection Science; Urumqi 830011; China）;
【作者单位】 新疆农业科学院土壤肥料研究所; 新疆城建环保有限公司; 新疆环境保护科学研究院;
【文献出处】 生物技术, Biotechnology, 编辑部邮箱 2009年 01期
期刊荣誉：ASPT来源刊 CJFD收录刊
【关键词】 筛选; 鉴定; 纤维素酶; 蜡质芽胞杆菌;
【英文关键词】 screen; identification; cellulase; Bacillus cereus;
【摘要】 目的:分离堆肥中具有产纤维素酶酶活的菌株并进行鉴定,同时进行该菌株纤维素酶酶特性的研究。方法:采用刚果红平板法进行筛选得到菌株,利用16SrDNA通用引物对其基因组DNA进行扩增,测序得到GCH-1的部分16SrDNA序列,经Blastn调出与菌株16SrDNA同源的序列,用软件MEGA 4.0按照Neighbor-Joining方法构建16SrDNA系统发育树,并采用DNS法测定所产纤维素酶酶活。结果:GCH-1的生理生化指标与蜡质芽胞杆菌理化指标相符,与Bacillus cereusIAM 12605(T)处于同一分支,相似性为99.8%.GCH-1菌株发酵48h能达到最大产酶量,所产纤维素酶在40℃有最高酶活力,酶活力分别为0.581u/mL,GCH-1菌株产生的纤维素酶酶系在pH 7.0~9.0能够保持较高的相对酶活力,超过85%相对活力。结论:GCH-1初步鉴定为蜡质芽孢杆菌(Bacillus cereus),能够产胞外纤维素酶。
【英文摘要】 Objective:To screen the bacteria of producing cellulase in composite and identify,and study the characteristics of cellulase.Method: The gangguo red plate method was implemented to screen the bacteria that were named as GCH-1;the part 16S rDNA was PCR using universal primer and sequenced.The 16S rDNA phylogenetic tree was constructed by comparing with published 16S rDNA sequences of relative bacteria species using Neighbor-joining,and DNS method was used to analyze enzyme activity.Result: It was main physio...
【基金】 新疆自治区高技术研究与发展计划项目(200715114);; 乌鲁木齐市科技计划项目(G07132002)资助
【DOI】 CNKI:SUN:SWJS.0.2009-01-013
