【英文篇名】 Effects of Main Nutrients on Glucoamylase and Protease Activity Produced by Aspergillus Niger
【作者中文名】 肖雷; 冷云伟; 陶秀祥; 刘炯天; 王璐璐;
【作者英文名】 XIAO Lei; LENG Yun-wei; TAO Xiu-xiang; LIU Jiong-tian; WANG Lu-lu（School of Chemical Engineering and Technology; China University of Mining & Technology; Xuzhou; Jiangsu 221116; China）;
【作者单位】 中国矿业大学化工学院;
【文献出处】 中国矿业大学学报, Journal of China University of Mining & Technology, 编辑部邮箱 2009年 01期
期刊荣誉：中文核心期刊要目总览 ASPT来源刊 中国期刊方阵 CJFD收录刊
【关键词】 黑曲霉; 糖化酶; 蛋白酶; 营养成分;
【英文关键词】 aspergillus niger; glucoamylase; protease; nutrients;
【摘要】 考察了培养基的配比、部分简单碳源对黑曲霉AS3.350产糖化酶、蛋白酶和孢子收获量的影响.结果表明:在选择的2种有机氮源中,豆粕可被黑曲霉较好地利用.在麸皮与豆粕的质量比为5∶5,30℃培养72 h时,糖化酶活力最高为1 185 U/g,蛋白酶活力差异不显著.在分别添加葡萄糖0.5%,蔗糖0.75%时,黑曲霉产糖化酶酶活力达到最高;对添加淀粉来说,随着淀粉浓度的增高而糖化酶活力逐渐增大.蛋白酶活力和孢子收获量与碳源的种类、浓度以及菌体的生长情况密切相关.
【英文摘要】 The effects of some medium components,including culture medium proportions and several simple carbon sources,on protease and glucoamylase activity of aspergillus niger were investigated.The results show that among two kinds of organic nitrogen sources soybean meal could be used efficiently by aspergillus niger,the optimal conditions of culture medium for enzyme secretion was the mass ratio of wheat bran and soybean meal of 5∶5 and the glucoamylase activity of 1 185 U/g,when the cultivating condition was 20 ...
【基金】 国家高技术研究发展计划(863)项目(2006AA102314);; 徐州市社会发展基金项目(xz06063)
【DOI】 CNKI:SUN:ZGKD.0.2009-01-030
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